Lang O. 1, Kohidai L. 1, Kohidai Z. 2, Dobo-Nagy C. 3, Csomo K.B. 4, Lajko M. 4, Mozes M. 5, Keki S. 6, Deak G. 7, Tian K.V. 8, Gresz V. 9.
Author information
1 Chemotaxis Research Group, Department of Genetics, Cell- and Immunobiology, Semmelweis University, Nagyvarad ter 4., H-1089 Budapest, Hungary.
2 Chemotaxis Research Group, Department of Genetics, Cell- and Immunobiology, Semmelweis University, Nagyvarad ter 4., H-1089 Budapest, Hungary; Department of Oral Diagnostics, Semmelweis University, Szentkiralyi utca 47., H-1088 Budapest, Hungary.
3 Department of Oral Diagnostics, Semmelweis University, Szentkiralyi utca 47., H-1088 Budapest, Hungary; Materials Science Research Institute, Faculty of Dentistry, Semmelweis University, Szentkiralyi utca 47., H-1088 Budapest, Hungary. Electronic address: dobo-nagy.csaba@dent.semmelweis-univ.hu.
4 Department of Oral Diagnostics, Semmelweis University, Szentkiralyi utca 47., H-1088 Budapest, Hungary.
5 Institute of Pathophysiology, Semmelweis University, Nagyvarad ter 4., H-1089 Budapest, Hungary.
6 Department of Applied Chemistry, University of Debrecen, Egyetem ter 1., H-4032 Debrecen, Hungary. Electronic address: keki.sandor@science.unideb.hu.
7 Department of Applied Chemistry, University of Debrecen, Egyetem ter 1., H-4032 Debrecen, Hungary. Electronic address: deak.gyorgy@science.unideb.hu.
8 Materials Science Research Institute, Faculty of Dentistry, Semmelweis University, Szentkiralyi utca 47., H-1088 Budapest, Hungary; Department of Chemical Science and Technologies, NAST Center, University of Rome Tor Vergata, Rome, Italy.
9 Department of Oral Diagnostics, Semmelweis University, Szentkiralyi utca 47., H-1088 Budapest, Hungary. Electronic address: greszveronika@gmail.com.
Abstract
The cytotoxicity of glass ionomer cements (GICs) was investigated using a novel, cost-effective, easy-to-perform and standardized test. GIC rings were made using in-house designed, custom-made moulds under sterile conditions; 10 with Fuji Equia and 10 with Fuji Triage capsules, placed in direct contact with primary human gingival fibroblasts (HGF) and immortalized human fibroblasts (HFF1). On day 1, 4, 14 and 21, an AlamarBlue® (resazurin) assay was completed towards determining the effects of the GICs on metabolic activities of the cells, whilst cell morphology was examined by light microscopy. The influence of the compounds released from the GIC rings on cell physiological effects (viability, proliferation and adhesion) during 24 h incubation was further investigated by impedimetry. Result trends obtained from this battery of techniques were complementary. At 100 v/v% concentration, the released compounds from Equia were strongly cytotoxic, while at lower concentration (0, 4, 20 v/v%) they were not cytotoxic. In contrast, Triage elicited only slightly transient cytotoxicity. The method proposed has been proved as being efficient, reliable and reproducible and may be useful in quick testing of the cytotoxicity of similar biomaterials by using an immortalized cell line.
Copyright © 2019. Published by Elsevier Ltd.
KEYWORDS:
Biomaterial; Cytotoxicity; Fibroblast; Glass ionomer cement; Histology
PMID: 31419507
DOI:10.1016/j.tiv.2019.104627
Toxicol In Vitro. 2019 Aug 13:104627. doi: 10.1016/j.tiv.2019.104627. [Epub ahead of print]